RNA Seq Library Prep Kit

The RNA Seq Library Prep Kit, compatible with Illumina and MGI platforms, enables the creation of high-quality NGS libraries for next-generation sequencing. RNA sequencing, a potent tool for transcriptome analysis, facilitates studies on gene expression, transcription regulation, splicing, mutations, and variations. The kit requires purified RNA, such as rRNA-depleted RNA or polyA mRNA, as input. Library multiplexing is achievable using various index types. The kit's workflow offers three index options for Illumina platforms: non-index, index, and unique dual index, each with distinct capabilities and sample multiplexing limits. Key features include a quick 2-hour protocol, minimal hands-on time, guaranteed high yield, uniform transcript coverage, and a simple workflow. The kit accommodates purified RNA inputs ranging from 3 ng to 100 ng. Comparative analyses demonstrate the kit's efficiency and uniform coverage. Additionally, it is part of a suite of related products.

• High-Quality NGS Library Construction: This kit facilitates the creation of superior quality libraries for next-generation sequencing on Illumina and MGI platforms. RNA sequencing, a potent tool, enables analysis of transcriptomes, including gene expression, transcription regulation, splicing, and mutation detection. Purified RNA (like rRNA-depleted or polyA mRNA) is required as input
• RNA Seq Library Prep Kit Workflow
• Index Options for Illumina Platforms:
• Non-index (illumina Cat.# 30055): No index present in libraries
• Index (illumina Cat.# 30056): Unique 6-base barcode in index primers, enabling multiplexing up to 48 samples. Index information available here
• Unique Dual Index (illumina Cat.# 30057): Up to 96-sample multiplexing, 4-Base Difference Index System for error identification. Index information available here
• MGI Platform Index Availability (Cat.# 34112)
• Kit Features
• Swift Protocol: Libraries ready in 2 hours
• Minimal Hands-on Time: 10 minutes
• Ensured Quality: High yield
• Uniform Transcript Coverage
• User-Friendly Workflow
• RNA Input Range: 3 ng to 100 ng
• Protocol Time Comparison: BioDynami vs Other Vendors